Melania Geymonat, 28, and partner Chris Hannigan were on a night bus in London on 30 May when they were allegedly targeted by four teenage boys.
It was alleged that the boys used homophobic language to insult the two women. They will stand trial on 28 November for allegedly asking them to perform lewd acts with each other on the bus.
All four are charged with using threatening, abusive, or insulting words or behaviour to cause harassment, alarm, or distress it has emerged.
It was also alleged the boys threw coins at the women, who were then taken to hospital for treatment to facial injuries after the alleged coins hit their faces.